| Registro completo |
| Provedor de dados: |
OAK
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| País: |
Japan
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| Título: |
DNA vaccine coding a p23 protein of Cryptosporidium parvum fused with Fc portion of immunogloblin G induces higher level of interferon-γ expression in mice
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| Autores: |
Takashima, Yasuhiro
Xuan, Xuenan
Shirafuji, Hiroaki
Zhang, Guohong
Otsuka, Haruki
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| Data: |
2005
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| Ano: |
2005
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| Palavras-chave: |
C. parvum
DNA vaccine
Fc
Interferon-8
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| Resumo: |
To develop DNA vaccines against Cryptosporidiosis, a plasmid coding an immunodominant protein of Cryptosporidium parvum sporozoite, p23 (pCX-p23) and another plasmid coding a fusion protein containing whole the p23 and the Fc portion of mouse immunogloblin G1 (pCX-p23Fc). Vaccination of BALB/c mice with the plasmid, pCX-p23 and pCX-p23Fc induced the production of antibodies against p23. Although both of splenocytes of mice immunized with the plasmids pCX-p23 and pCX-p23Fc expressed interleukin-4 and interferon-8, after the in vitro stimulation by p23 antigen, the interferon-8 expression level of pCX-p23Fc immunized mice was much higher than that of pCX-p23 immunized mice. These results suggest a possibility of the plasmid pCX-p23Fc as a DNA vaccine candidate against cryptosporidiosis.
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| Idioma: |
Inglês
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| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/147
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| Editor: |
National Research Center for Protozoan Deseases
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| Formato: |
application/pdf
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